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. 2012 Oct 10;34(1):28–37. doi: 10.1093/carcin/bgs316

Fig. 1.

Fig. 1.

Alcohol induces RNA Pol III-dependent transcription. (A and B) Ethanol enhances transcription in MCF-10A cells. MCF-10A cells were starved in DMEM/F12 for 3h. Cells were treated with or without different amounts of ethanol. (C and D) Ethanol induces transcription in ER− non-tumorigenic cells. MCF-10F, MCF-12A and MCF-10A cells were treated with or without 25mM ethanol; *P < 0.05, **P < 0.01 control compared with ethanol. (E and F) Ethanol stimulates transcription in MCF-7 cells. MCF-7 and MCF-10A cells were treated with or without 25mM ethanol. **P < 0.01 MCF-7 compared with MCF-10A. (G and H) Ethanol increases transcription in different breast cancer cell lines. ER+ breast cell line T-47D and ER− breast cell lines (MDA-MB231 and SKBR-3) were treated with ethanol. **P < 0.01 ER+ cancer cells compared with ER− cancer cells. RNA was isolated from these cells and RT-qPCR was performed to measure the amounts of pre-tRNALeu (A, C, E, G) and 5S rRNA (B, D, F, H). The fold change was calculated by normalizing to the amount of glyceraldehyde 3-phosphate dehydrogenase (GAPDH) mRNA. The bars represent mean ± SE of at least three independent determinations.