Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2012 Oct 10;12:187. doi: 10.1186/1471-2229-12-187

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright ©2012 Karan and Subudhi; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

Complementation of the yeast Ubc9-ts mutation by expression of the SaSce9. (a) Schematic representation of yeast expression vector (pVTL260-SaSce9) used for complementation assay, containing an ORF of SaSce9 cloned at NheI and BamHI site driven by ADH promoter; (b) Strains used in this experiment. Ubc9-Ts: temperature sensitive mutant for SUMO conjugating enzyme of Saccharomyces cerevisiae; pVTL260: Ubc9-Ts mutant carrying only vector as control; SaSce9: Ubc9-Ts mutant carrying pVTL260-SaSce9 of Spartina alterniflora; (c) Growth of yeast strains at 25°C; and (d) Growth of yeast strains at 37°C on solid yeast, peptone, and dextrose containing (YPD) media for 3 days.