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Specificity of the myelin basic protein (MBP) antibody in staining the brain striatum. The antibody was used at a dilution of 1:50 (A) and 1:500 (B). The control containing the secondary antibody alone (no MBP antibody) is shown in C. Using the optimal 1:50 dilution, the MBP antibody was preabsorbed with the MBP protein prior to staining. Panel D shows the MBP staining without the blocking protein, while panel E shows the effect of blocking by the protein. Bar = 50 µm.
