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Number of Ki67-positive hepatocytes in rabbit liver tissue sections with automated analysis versus visual counting by two observers in all images analyzed (n=100; i.e., five random fields in 20 tissue blocks at ×200 magnification). (A) Analysis of CK-Ki67 immunohistochemistry (IHC) double staining. (B) Analysis of CD31-CK-Ki67 IHC triple staining. Note the higher correlation coefficients after IHC triple-staining. ▲ = observer 1; □ = observer 2.
