Figure 6.

Identification of phenotypes of vascular endothelial growth factor receptor–3 (VEGFR-3)–expressing cells in the lumbar spinal cords at the onset stage of experimental autoimmune encephalomyelitis (EAE)–affected rats. (A–F) Triple labeling with VEGFR-3, ED1, and laminin showing that ED1-positve macrophages expressing VEGFR-3 (asterisks in E and F) were often associated with laminin-positive vascular profiles. (E, F) Higher magnification views of the boxed areas in A–C, respectively. (G–J) Double labeling using VEGFR-3 and CD45 showing that nearly all of CD45-positive cells coexpressed VEGFR-3. (H–J) Higher magnification views of the boxed area in G. Scale bars = 100 µm for A–D, G; 50 µm for H–J; 20 µm for E, F.