Figure 2. Demonstration of dsRNA transmission from adult bee to Varroa via the bee hemolymph.

Northern blot assay was performed on RNA extracted from a bee that had ingested dsRNA-GFP (B+) and from an untreated bee (B−), pooled RNA extracted from hemolymph collected from bees that had ingested dsRNA-GFP (H+) and from untreated bees (H−), and pooled RNA extracted from Varroa mites parasitizing dsRNA-GFP-treated bees (V+) and untreated bees (V−). C = positive control (GFP-carrying plasmid).