Table 2.
reference sequence: AA | |||||
---|---|---|---|---|---|
native 454: AAA | |||||
|
qs 1 |
qs 2 |
qs 3 |
qs 4 |
qs 5 |
QS454 |
22 |
22 |
23 |
- |
- |
HPCall: AAA | |||||
|
HPL 1 |
HPL 2 |
HPL 3 |
HPL 4 |
HPL 5 |
|
1.7E-10 |
0.29 |
0.71 |
3E-9 |
<1E-15 |
QSHPCall | 0 | -1 | 5 | 0 | 0 |
Base-calling probabilities example 2: overcall. HPCall gives a probability for HPL 3 of 0.29 and an associated QSHPCall of 5. This indicates that it is not unlikely that the fourth called A is an overcall. This information can not be extracted from the quality scores of the native 454 base-caller.