Table 2.
Hydrogenase activity induced by R. leguminosarum strains in microaerobic cultures and in pea bacteroids
|
Strain |
Genotype |
Hydrogenase activitya |
||
|---|---|---|---|---|
| 1% O2 | 3% O2 | Bacteroids | ||
| UPM1155(pALPF1) |
wild type |
13440 ± 1720 |
4970 ± 472 |
5980 ± 852 |
| UPM1155 (pALPF14) |
ΔhypC |
< 200 |
< 200 |
< 200 |
| UPM1155 (pALPF5) |
ΔhupF |
< 200 |
< 200 |
470 ± 84 |
| UPM1155 (pALPF5/pPM501) |
ΔhupF/hupFST |
12890 ± 230 |
5900 ± 779 |
5350 ± 728 |
| UPM1155(pALPF5/pPM501C) | ΔhupF/hupFCST | 4780 ± 228 | 1025 ± 255 | 5400 ± 683 |
aValues, expressed in nmoles H2 h-1 (mg protein)-1, are the average of three independent assays ± SE.