. 2012 Nov 8;12:256. doi: 10.1186/1471-2180-12-256

Table 4.

Oligonucleotides used in this work

Primer	Sequence (5^′-3^′)	Use
HUPF5	GCGGCACTGCTGGTCGGCTGAATCACTCCCAAGGCTGAGGTGTAGGCTGGAGCTGCTTC	hupF deletion
HUPF3	CGGCGGCAATTCCGGCTCGCGGGAAATGAGATCGGCGAACATATGAATATCCTCCTTAGT	hupF deletion
HUPK52	ACATTCCTTCTCGGGGCCGGCACGATAGGGATCGACGTGATTCCGGGGATCCGTCGACC	hupK deletion
HUPK32	TGCGATAGGCTGCGAGCCTGCCGTCACCGCCGATTTCGAGTGTAGGCTGGAGCTGCTTC	hupK deletion
HYPC5	GAACGAACATGCATCAATCGAGGAGAACCGGACATGTGCATTCCGGGGATCCGTCGACC	hypC deletion
HYPC3	TCGTCGATATATTTCATGCCGCATCTCCCATCGCACGTTGTGTAGGCTGGAGCTGCTTC	hypC deletion
TAGF31	TCCCGCGAGCCGGAATTGCCGCCGCATTTGCGCGGCGAGTGGAGCCACCCGCAGTTCGA	Generation of hupF_ST fusion (pALPF382)
TAGF32	GGTGGAAACTCAAATTCCATTTTGGAAGTTCTCTTTTCAATATGAATATCCTCCTTAGT	Generation of hupF_ST fusion (pALPF382)
FNDE	CTGTCAGTCGTCATATGTGCATCGGCATTCCCAT	Generation of pPM501/pPM501C plasmids
MANG3	ACGGCGGCGGGAATGCTC	Generation of pPM501/pPM501C plasmids
HUPF-3413 L-Strep	GAGTACTCTCAGGCGCCTTTTTCGAACTGCGGGTGGCTCCAGCTAGCTCCCTCCACCGCTTCGGCAAGTCCGGCGAG	Generation of pPM501C plasmid