Table 1.
Primer pairs used for direct bisulfite sequencing and combined bisulfite restriction analysis of hypermethylated genes
| Gene | GenBank Accession No. | Primer (5' → 3') | |
|---|---|---|---|
|
PITX2 |
NM_000325.5 |
F |
TAGTGATAGGCGTTTCGGGTT |
| |
|
R |
CCACTACATACTAACAAACACTCAAAT |
|
TLX3 |
NM_021025 |
F |
TCGGTTGAGGATTAGAGGGATT |
| |
|
R |
AACGCCACCTAACCATCTATTC |
|
OTX2 |
NM_172337.1 |
F |
AGTTGTGTTAGGTTGAGGGAG |
| |
|
R |
AATCCCAAAAACCTTTTTAAA |
|
MNX1 |
NM_001165255 |
F |
TTTAAGAAATAGCGAGAGGGAG |
| |
|
R |
AAACGCTCGTAACATAATCCC |
|
NR2F2 |
NM_001145155 |
F |
CGTATCGTGGATTTGGAGTAGGGTATT |
| |
|
R |
AACAAACTCGCTAACAAATAAACRACATT |
|
TFAP2C |
NM_003222 |
F |
CGAAGTGTTAGGGTTTTGTGTGT |
| R | CGACCTTAAACAACAACCAAATCC |