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. 2012 Nov 8;15(1):29–40. doi: 10.1093/neuonc/nos248

Fig. 2.

Fig. 2.

NP-TRAIL induces apoptosis and decreases self-renewal of GSCs with and without γ-radiation and bortezomib. The GSCs HF2414 (A and C) and HF2485 (B and D) were treated with TRAIL (20 or 100 ng/mL) or with NP-TRAIL at similar concentrations. Cell apoptosis was determined after 48 h using PI staining and FACS analysis (A and B). Neurosphere formation assay was performed, and the number of neurospheres per well was quantified after 14 days for eight different wells (C and D). Spheres that contained more than 20 cells were scored, and the results are presented as percentage of maximal neurospheres formed in control untreated cells. For combined treatment of NP-TRAIL with γ-radiation (E and G) and bortezomib (F and G), cells were radiated with 3Gy and NP-TRAIL was added 6 h later. Bortezomib (100 nM) and NP-TRAIL (100 ng/mL) were added concomitantly. Neurosphere formation was determined after 14 days (E and F), and the morphology of the control and treated HF2414 GSCs was visualized after 3 days under a phase contrast microscope. All images are 20X (G). Results are representative of 3 different experiments or are the mean ± standard error of the mean of 3 experiments. *P < .001 **P < .05.