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. 2012 Nov 8;15(1):29–40. doi: 10.1093/neuonc/nos248

Fig. 4.

Fig. 4.

FL-NP-TRAIL localize to tumor cells in U251 xenografts. Rhodamine-labeled control NP (FL-NP) or NP conjugated to TRAIL (FL-NP-TRAIL, 0.25 μg) were implanted directly within the tumor mass 7 days after GFP-U251 tumor cell implantation. Four days later, animals were euthanized, brains harvested and snap frozen for sectioning and imaging. As can be seen in the bottom panel, the FL-NP-TRAIL were not only found in areas of tumor mass, but they also colocalized within tumor cells (I), whereas the control NP (FL-NP) were found nearby, but did not colocalize with tumor cells (F). No red fluorescence was observed in the PBS-treated animals (C). All images are 20X. (A–C) – PBS treated; (D–F) – NP treated; (G–I) – NP-TRAIL treated. (A, D, G) – red channel; (B, E, H) – green channel; (C, F, I) – merged red and green channels. The results are representative of three different experiments that gave similar results.