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Gene maps of expression vectors pET101-HO1 (A) and pJexpress401-mHO1 (B). ho1 gene expression in (A) is controlled by T7lac promoter which consists of a strong bacteriophage T7 promoter and a downstream 25 bp lac operator in pET101. For mho1 expression (B), an “IP-free” T5 promoter sequence was used with the lac operator placed downstream of the T5 promoter in the vector pJexpress 401.
