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. 2012 Oct 23;2:56. doi: 10.1186/2191-0855-2-56

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Copyright ©2012 Nakanishi et al.; licensee Springer.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fluorescence observation of RTSH yeast, harboring EG with CBM having RTSH mutation, CBH, and BG, after immunofluorescence labeling. Yeast cells were labeled with the following antibodies: mouse monoclonal anti-FLAG M2 antibody for EG (upper column), mouse monoclonal StrepMAB-Classic antibody for CBH (middle column), mouse monoclonal anti-RGS-His antibody for BG (lower column), and Alexa Fluor 488 anti-mouse immunoglobulin. The left column represents RTSH yeast and the right column represents yeast harboring pRS423, pRS425, and pRS426 (control vectors). Phase-contrast micrographs are presented in the left column and fluorescence micrographs are presented in the right column. The scale bar is 5 μm.