Table 2.
Primers to construct the comprehensively mutated CBM and to confirm the mutated CBM-sequences
| Primer | Sequence |
|---|---|
| pEGout F |
5’-CGTAGGTCCGCTCCAACCAATACC-3’ |
| pEGout R |
5’-CTCAATCCTTATTATGCGCAATGTATTCCG-3’ |
| NNK primer |
5’-TTGGAGCGGACCTACGNNKTGTGCTCCTGGCNNKGCTTCTNNKNNKCTCAATCCTTATTATGCGCAATGT-3’ |
| NNK rev |
5’-ACATTGCGCATAATAAGGATTGAG-3’ |
| EGct F1 |
5’-CCAGTGTGGAGGTATTGGTTGGAGCGGACCTACG-3’ |
| EGct R1 |
5’-GTCGAAGTGGTGATAGTAGTGGCTCCCGGAATACATTGCGCATAATAAGGATTGAG-3’ |
| EGct F2 |
5’-ATAGATCTCAGCAGACTGTCTGGGGCCAGTGTGGAGGTATTGG-3’ |
| EGct R2 |
5’-GCACGCGTGGTGGTGGTTGGACCGGATGGTGGCCGGGTCGAAGTGGTGATAGTAGTGGC-3’ |
| CHK |
5’-CATGCAACTGTTCAATTTGCCATTG-3’ |
| EGII360bp rev | 5’-AGGATAAACCTTCGAGGTAACGCAAGTGCC-3’ |