Table 6.
The PiceaGenExpress database: sample characteristics, hybridizations and detected genes
| |
Plant material |
Microarrays |
Genes5 |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Tissue type | Sp1 | Source sampling2 | Genotype3 | Slide | Imaging4 | Total | Unique | Non-annotated | |
| 1 |
Embryogenic cells |
Pgl |
a, a |
1 |
6 |
SQ |
10,066 |
100 |
2,456 |
| 2 |
Vegetative buds |
Pgl |
b, c |
2 |
10 |
SQ |
12,361 |
128 |
3,216 |
| 3 |
Xylem (Mature) |
Pgl |
d, d |
60 |
60 |
SQ |
14,686 |
176 |
4,232 |
| 4 |
Xylem (juvenile) |
Pgl |
e, f |
30 |
20 |
SQ |
13,807 |
56 |
3,701 |
| 5 |
Phelloderm |
Pgl |
e, f |
30 |
20 |
SQ |
15,803 |
214 |
4,391 |
| 6 |
Young needles |
Pgl |
e, f |
30 |
10 |
SQ |
12,819 |
167 |
3,025 |
| 7 |
Megagametophytes |
Pgl |
g, c |
3 |
3 |
PA |
17,056 |
1,111 |
5,205 |
| 8 |
Adventitious roots |
Pab |
h, c |
8 |
20 |
PA |
15,718 |
393 |
4,696 |
| |
|
|
|
|
Total detected |
21,241 |
2,345 |
|
|
| Not detected | 2,612 | ||||||||
1 Pgl: Picea glauca (White spruce); Pab: Picea abies (Norway spruce).
2 The source of materials and the sampling method are from the following studies: a, [43]; b, [37]; c, this paper, see methods; d, [23]; e, O-P seedlot, this paper see methods; f, [22]; g, C2856 parent from [38]; h, [44].
3 Total numbers of genotypes analyzed (either individually or in pools).
4 Microarray Scanner and Image Processing: SQ, ScanArray Express (Perkin Elmer, Waltham MA, USA) and QuantArray v3.0 (Packard BioChip Technologies, Billerica, MA, USA); PA, PowerScanner (Tecan Group Ltd., Männedorf, Switzerland) and ArrayPro Analyser v6.3 (Media Cybernetics, Bethesda, MD, USA).
5 Total: number of genes above background (see methods); Unique: genes detected only in one tissue.