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. 2013 Jan 2;8(1):e53462. doi: 10.1371/journal.pone.0053462

Figure 1. In vivo BLM imaging of infection progress using WEEV.McM.FLUC.

Figure 1

A: 24 hpi B: 48 hpi C: 72 hpi D: Survival analysis of WEEV.McM.FLUC and wild-type virus (WEEV.McM) from subcutaneous and intranasal virus challenge experiments. Note uniform lethality resulting from intranasal exposure by WEEV.McM.FLUC. E: FLUC activity was quantitatively measured in each animal at 1, 2, and 3 d.p.i. time points. Results from BLM analysis demonstrate robust FLUC activity as infection progressed with the greatest increase observed between days 2 and 3 post-infection. F: Brains of animals infected with WEEV.McM attain a higher viral titer more rapidly when compared with WEEV.McM.FLUC. WEEV.McM.FLUC titers approach that of McM by 72 h.p.i. G: Regression analysis of viral titer versus FLUC activity. Linear regression line appears curved due to log10 scaling of axis as required to clearly depict all data points (R2 = 0.9807).