Table 1. Caragana intermedia candidate reference genes descriptions and comparison with Arabidopsis orthologs.

Gene symbol	Gene name	GenBankAccession	Arabidopsishomolog locus	Primer sequence (5′–3′)	Ampliconlength (bp)	Tm(°C)	PCRefficiency (%)
ACT7	Actin7	JX272637	AT5G09810	CCGAAGAGCATCCAGTTTTG	58	78.5	100.67
				TCACGGTTAGCCTTGGGGTT
EF-1α	Elongation factor -1α	JX272638	AT5G60390	AGATGGTTCCCACTAAGCCTATG	115	82.6	93.13
				ACACTCTTGATGACTCCAACTGC
TUA5	Alpha Tubulin	JX272639	AT5G19780	CTGATGTGGTTGTGCTTTTGGAC	183	80.8	93.11
				GGTTTGTCTGGAACTCGGTAATG
F-box	F-box/kelch-repeat protein	JX272640	AT5G15710	AATGGGTCGTGGAGGGTCTA	71	82.3	94.80
				AACCCCTTGGCTTGGTCTTA
PEPKR1	Phosphoenolpyruvate carboxylase-related Kinase 1	JX272641	AT1G12580	GAACAGTTGGGTTGGGGACA	181	82.4	94.05
				GATCCACAACATTCGGGTGC
PP2A	Protein phosphatase 2A	JX272642	AT1G10430	TTTCGGATAGGAGGAAATGCAC	106	80.3	94.18
				TCAAGGCCACCAAAAGCGTA
SAND	SAND family protein	JX272643	AT2G28390	ATACTCGTCAACAGCAGAAA	133	79.8	91.46
				GTCACCCAACATAAAAGAAC
TIP41	TIP41-like family protein	JX272644	AT4G34270	CGTCCAAGAGTGGGAACAGA	167	81.7	90.22
				GAACTTCAACAGGCGGCAAG
UNK1	Hypothetical protein	JX272645	AT1G31300	CAATGTTGAGTGGGGAGGGA	162	81.2	97.41
				CAACCAGCCAAGCAAGGAAT
UNK2	Hypothetical protein	JX272646	AT4G33380	CAAAGATAGTGCTGCTGATTGC	147	81.6	92.64
				TCCTGGTGTTTGTGCTGATAGA
DREB1*	Dehydration responsive element binding protein 1	KC123242	AT4G25480	TTCTGACCCACAACCTTACTC	167	86.5	96.40
				TCTTCTTGTTTGGTTCCCTTA
DREB2*	Dehydration responsive element binding protein 2	JX272647	AT2G40340	GAAAGGGTGTATGAAAGGTA	228	83.4	92.56
				GTTATGTGAGGGAAGTTGAG

Notes: * used for normalization validation under cold or salt stress conditions.