Figure 2. Phospho-Akt is expressed in BrdU positive cells and over centrosomes of dividing cells in retinal monolayer cultures.

(A–C) Cultures at E7C1 were incubated with BrdU for 6 h, washed and cultivated for additional 15–16 h in fresh medium. Immunoassays were performed against phospho-Akt (A) and BrdU (B). Merged figures in C. Immunoassays of cultures at E7C1 performed against phospho-Akt (D) and gamma-tubulin (E). Merged figures in F. (G–J) High magnification micrographs showing double labeling against phospho-Akt and gamma-tubulin in dividing cells at prophase (G), metaphase (H), anaphase (I) and telophase (J). Nuclei were stained with DAPI in F–J. Arrow points to double labeled pAkt, γ-tubulin positive cells. Scale bar  = 10 µm in A–C and G, H, I, J and 20 µm in D–F.