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. 2013 Jan 2;8(1):e53517. doi: 10.1371/journal.pone.0053517

Figure 4. PI3K inhibition decreases phospho-Akt and phospho-GSK-3 levels in retinal cells.

Figure 4

Retinal explants from 7-day-old chick embryos were treated with 25 µM LY 294002 for 4, 8 or 24 h and protein extracts processed for detection of phospho-Akt and phospho-GSK3 expression. Gel loading was assessed with anti-Akt antiserum. Blots were quantified by densitometry and data are expressed as the mean ± S.E.M. of arbitrary units. **p<0.01 and ***p<0.001 relative to control. In both cases, representative blots from at least 3 independent experiments are shown. Ct  =  control explants cultivated without drug.