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. 2013 Jan 2;8(1):e53259. doi: 10.1371/journal.pone.0053259

Figure 6. Phg1A and Phg1B play redundant roles in controlling intracellular killing and Kil1 stability.

Figure 6

A) The amount of cell-associated lysozyme activity was measured as described in figure 2. As observed previously for lysosomal glycosidases [3], the cellular activity of lysozyme was reduced in phg1B KO cells compared to WT cells, and enhanced by Phg1B overexpression in phg1A KO cells. B) WT or mutant cells were grown on a lawn of Klebsiella bacteria. Loss of Phg1B was not sufficient to inhibit growth on Klebsiella, but overexpression of Phg1B in phg1A KO cells restored growth on bacteria. C) The ability of WT and mutant Dictyostelium cells to kill Klebsiella bacteria was measured, revealing that Phg1B overexpression restores efficient killing in phg1a KO cells (average and S.E.M. of 4 experiments). D) The total cellular amount of Kil1 was measured in WT and mutant cells. The intracellular level of Kil1 was increased in phg1a KO cells by overexpressing Phg1B. Average of at least 4 experiments. *: significantly different (Student t-test; p<0.05).