Figure 4. Quantitation of motility of HM18 and DI15 in INP0404 and INP0405 relative to ΔfliHI/flhB*.
Quantitative motility assays were performed for the isolated mutants in the presence of A) INP0404 and B) INP0405. The motility of each strain is expressed as a ratio of its motility in either drug relative to DMSO. Dots represent individual colonies. Lines represent means. For INP0404, an Kruskal-Wallis test indicated a difference between groups (p = 0.0017) and a Dunn's post-test indicated that only HM18 (p<0.001) was significantly more motile than ΔfliHI/flhB*. For INP0405, a Kruskal-Wallis test indicated a difference between groups (p<0.0001) and a Dunn's post-test indicated that HM18 (p<0.001) and DI15 (p<0.05) were significantly more motile than ΔfliHI/flhB*.