Table 4. Summary of results of genomic analysis performed.
Chromosomal location of single nucleotide change (SNC)a | Present in strain | Nature of change | Position relative to nearby genes | Identification of gene affected | Likely consequence on protein product |
282402 | ΔfliHI/flhB* | C→A | Intergeneic region between pseudogene cutF and proS (prolyl-tRNA synthetase) | None | NA |
1248480 b | HM18 | T→C | Intergeneic region between dinI (DNA damage inducible protein I and pyrC (dihydroorotase) | None | NA |
4080164 | HM18 | C→A | NA | atpB (F0F1 ATP synthase subunit A) | Disruption of start codon, new in frame ATG 12 nt down stream, truncation of 5 amino acids of predicted periplasmic N-terminus of AtpB |
4080165 | HM18 | C→A | NA | atpB (F0F1 ATP synthase subunit A) | Likely not translated due to SNC 4080164, new in frame ATG 11 nt down stream |
4189753 | HM18 | G→A | NA | fadA (3-ketoacyl-CoA thiolase, also known as multifunctional fatty acid oxidation complex subunit α) | Stop codon introduced, last 147 C-terminal amino acids missing |
2008607 | DI15 | C→G | NA | flhA (core inner membrane component of the flagellar export apparatus) | Single amino acid change introduced, A562P |
Defined by whole-genome Illumina sequencing and comparison to the Salmonella Typhimurium LT2 chromosome (RefSeq:NC_003197) and the 100-Kb plasmid pSLT (RefSeq:NC_003277). No differences were found between the plasmid of the LT2 reference and the two test strains.
Note that this mutation (but no other) was also found in HM16 and DI13, which are not further described here, but not in ΔfliHI/flhB*. We thus assume it to be phenotypically silent.