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. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Mol Cell Endocrinol. 2009 Jul 1;311(1-2):126–134. doi: 10.1016/j.mce.2009.06.011

Figure 2. Effect of kisspeptin on GnRH secretion in GT1-7 and GN11 cells.

Figure 2

GT1-7 and GN11 cells were incubated with 10−9M kisspeptin (30min through 6 hours) and GnRH levels in the medium were determined by RIA. The cellular total protein lysates from each treated culture dish was used to normalize the results. (A and D) Static incubation, (B and E) Dynamic incubation and (C and F) Total GnRH secretion in both kisspeptin and NT groups in GT1-7 and GN11 cells. Graphic representation of the means±SE of GnRH levels. Student’s t test was used for statistical analysis. Asterisks indicate significant differences (P ≤0.05).