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. Author manuscript; available in PMC: 2013 Jan 3.
Published in final edited form as: Mol Cell Endocrinol. 2009 Jul 1;311(1-2):126–134. doi: 10.1016/j.mce.2009.06.011

Figure 5. GnRH mRNA expression in GT1-7 cells treated with kisspeptin, MAPK inhibitor and PI3K inhibitor (quantitative RT-PCR).

Figure 5

(A) GT1-7 and (B) GN11 cells. Graphic representation of the means±SE of relative mRNA levels graphed as fold increasing of levels relative to non-treated cells. Cells were treated with 10−9M kisspeptin (Ks 10−9M), 10µM MAPK inhibitor or 50µM PI3K inhibitor. And co-treated with Ks 10−9M+MAPKi or Ks 10−9M+PI3Ki. There is no statistical difference between groups with the same letters (n=4–6, P ≤0.05; ANOVA followed by Newman–Keuls test).