TABLE 3.

Molecular Analyses Reveal the Presence of Independent Genetic Events in a Subset of Multifocal Non-invasive IPMN lesions

Patient Age, Gender	Lesion	IPMN Diagnosis	Subtype	KRAS	LOH-Analysis				Presence of Multiclonality
					D6S1040	D6S1009	D17S974	D17S1298
IPMN1 62f	N			Wt					Yes
	T1	BD-low-grade	Gastric	G12D		MSI	MSI	MSI
	T2	BD-low-grade	Gastric	G12V, G12D
IPMN2 71m	N			Wt				Na	Yes
	T1	BD-intermediate	Gastric	G12D, G12V				Na
	T2	BD-low-grade	Gastric	G12V				Na
IPMN3 69m	N			Wt					Yes
	T1	BD-low-grade	Gastric	G12V
	T2	BD-intermediate	Gastric	G12D
IPMN4 54f	N			Wt			Na		Yes
	T1	BD-low-grade	Gastric	Wt		MSI	Na
	T2	BD-intermediate	Gastric	G12V			Na
	T3	BD-low-grade	Gastric	G12V			Na
IPMN5 69m	N			Wt					Yes
	T1	BD-low-grade	Gastric	G12D
	T2	BD-high-grade	Gastric	G12D
	T3	BD-intermediate	Gastric	G12V
	T4	BD-low-grade	Gastric	G12R, G12D	MSI		MSI	MSI
	T5	BD-low-grade	Gastric	G12D, G12R
IPMN6 66f	N			Wt			Na	Na	Yes
	T1	BD-low-grade	Gastric	G12R			Na	Na
	T2	BD-intermediate	Gastric	G12R, G12D			Na	Na
IPMN7 71f	N			Wt	Na	Na	Na	Na	Indeterminate
	T1	MT-high-grade	Intestinal	Wt	Na	Na	Na	Na
	T2	MT-high-grade	Intestinal	Wt	Na	Na	Na	Na
IPMN8 68m	N			Wt	Na	Na	Na	Na	No (cysts likely related)
	T1	MT-low-grade	Gastric	G12D	Na	Na	Na	Na
	T2	MT-high-grade	Gastric/intestinal	G12D	Na	Na	Na	Na
IPMN9 66m	N			Wt					Yes
	T1	MT-high-grade	Oncocytic	Wt
	T2	MT-high-grade	Oncocytic	Wt
IPMN10 73f	N			Wt					No (cysts likely related)
	T1	BD-low-grade	Gastric	G12D
	T2	BD-high-grade	Oncocytic/gastric	G12D
IPMN11 69f	N			Wt					No (cysts likely related)
	T1	BD-intermediate	Gastric	G12V
	T2	BD-intermediate	Gastric	G12V
IPMN12 70m	N			Wt	Na	Na	Na		Yes
	T1	MT-high-grade	Gastric	G12D	Na	Na	Na
	T2	BD-low-grade	Gastric	G12D, G12R	Na	Na	Na
IPMN13 50m	N			Wt	Na	Na		Na	Yes
	T1	MD-high-grade	Intestinal	Wt	Na	Na		Na
	T2	MD-high-grade	Intestinal	G12V	Na	Na		Na

Both alleles present.

Loss of longer allele.

Loss of shorter allele.

LOH analysis using 4 polymorphic tetranucleotide microsatellite markers was performed with allelic ratio cutoffs of <0.59 and >1.70.

MSI indicates microsatellite instability; N, matched normal tissue; Na, noninformative (single allele) or failed to amplify after multiple attempts; T, tumor tissue; Wt, wild type.