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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: Biochim Biophys Acta. 2012 Nov 28;1832(2):375–385. doi: 10.1016/j.bbadis.2012.11.012

Figure 6. The effect of “KK” loop mutation on FUS subcellular localization.

Figure 6

The GFP-tagged wild-type full-length FUS (top), truncated FUS without the C-terminal NLS (FUS-ΔNLS, middle) and the “KK” loop mutant (FUS-ΔNLS-AAA, bottom) were transfected into N2a cells. Representative confocal microscopic images of GFP-FUS and DAPI-stained nucleus are shown. The “KK” loop mutation reversed the cytoplasmic accumulation of FUS-ΔNLS and showed even distribution in both nucleus and cytoplasm. The scale bar is 10 μm.