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. 2012 Nov 28;32(48):17391–17400. doi: 10.1523/JNEUROSCI.2438-12.2012

Figure 3.

Figure 3.

FAIRE showing a time course Kiss response. Kiss induces opening of chromatin within the KsRE of the GnRH promoter in GT1–7 cells. A, FAIRE analysis at the KsRE contained between −3446 and −2806 bp on the mGnRH promoter (red shading). B, FAIRE at the nKsRE contained between −2418 and −2413 bp on the mGnRH promoter located in the GnRHen (green shading). C, FAIRE performed at the NSE (blue shading) of the mGnRH promoter. D, Control of nonactive chromatin of the GnRH promoter contained between −1858 and −1567 bp (black line). Graphic representation of the means ± SE of relative DNA (qRT-PCR) graphed as fold increase of levels relative to NT group. Cells were treated with 10−9 m Kiss for 15 and 45 min (n = 3, p ≤ 0.05). There is no statistical difference between groups with the same letters.