Table 1.

Sequence of nucleotides

Primers	DNA sequences
qRT-PCR GnRH sense	5′ CCCTTTGACTTTCACATCC 3′
qRT-PCR GnRH antisense	5′ GGGTTCTGCCATTTGATCCAC 3′
qRT-PCR Otx2 sense	5′-GCAGAGGTCCTATCCCATGA-3′ (Larder and Mellon, 2009)
qRT-PCR Otx2 antisense	5′-CTGGGTGGAAAGAGAAGCTG-3′ (Larder and Mellon, 2009)
qRT-PCR 18S sense	5′-TGGTTGATCCTGCCAGTAG-3′
qRT-PCR 18S antisense	5′-CGACCAAAGGAACCATAACT- 3′
Otx-2 mutant sense	5′-GCGAGAATTACCGGCGCCAAAGAACTTTTTCG-3′
Otx-2 mutant antisense	5′-GCTTTTTCAAGAAACCGCGGCCATTAAGAGCG-3′
Otx-2 binding site at the KsRE (−3005/−3000 bp)
Sense	5′-CAGAGCTGATGGAAACCACA-3′
Antisense	5′-AGGAAACCACACCCAACAAG-3′
Otx-2 binding site at the nKsRE (−2418/−2413 bp)
Sense	5′-GGCTTGATTATAGCCACCAGGAAAGG-3′
Antisense	5′-GACATGCCACTCTCCTGA-3′
Otx-2 binding site at the NSE (−319/−252 bp)
Sense	5′-TCCCTGTGAGTTTTTCAGTGTG-3′
Antisense	5′-GCAGCACAGCCCATAGTCTT-3′
Negative control primers (−1858/−1567 bp)
Sense	5-′TGAGATGAACAGCCCACAAA-3′
Antisense	5′-TGCTCAGTCCTCATGACAGG-3′

Mutated sequences are underlined.