Table 1.
Antibody information
| Antibody | Company | Purpose | Dilution |
|---|---|---|---|
| Ac-Histone H3 | Santa Cruz | Western Blot | 400 |
| Ac-p53 K379 | Cell signaling | Western Blot, IF | 1000 |
| Ac-p53 K386 | Abcam | Western Blot, IF | 1000 |
| Akt 1/2/3 H-136 | Santa Cruz | Western Blot | 400 |
| Bax | Cell Signaling | Western Blot | 1000 |
| Bcl2 | Cell signaling | Western Blot | 1000 |
| Cleaved caspase-3 | Cell signaling | Western Blot | 1000 |
| Cyclin D1 | Santa Cruz | Western Blot | 500 |
| Cyclin D2 | Santa Cruz | Western Blot | 500 |
| Cyclin A | Santa Cruz | Western Blot | 500 |
| Cyclin E | Santa Cruz | Western Blot | 500 |
| E-cadherin | Santa cruz | Western Blot | 500 |
| Fibronectin | Abcam | Western Blot | 1000 |
| HDAC-1 | Santa Cruz | Western Blot, IHC | 500 |
| HDAC-2 | Santa Cruz | Western Blot, IHC | 500 |
| HDAC-3 | Santa Cruz | Western Blot | 500 |
| HDAC4/5/7 | Santa Cruz | Western Blot | 500 |
| HDAC-6 | Santa Cruz | Western Blot | 400 |
| N-cadherin | Santa Cruz | Western Blot, IF | 500 |
| PCNA | Santa Cruz, | Western Blot | 500 |
| PCNA | Abcam | IHC | 500 |
| Phospho Akt1/2/3 thr-308 | Santa Cruz | Western Blot | 200 |
| Phospho Akt1/2/3 ser-473 | Santa Cruz | Western Blot | 200 |
| Phospho- P-S6 Ribosomal protein Ser-235/236 | Cell Signaling | Western Blot | 1000 |
| phospho-P70 S6 kinase Thr-389 | Cell Signaling | Western Blot | 1000 |
| Phospho-4E-BP1 Ser-6 | Cell Signaling | Western Blot | 1000 |
| Phospho 44/42 MAPK | Cell Signaling | Western Blot | 1000 |
| P-44/42 MAPK (ERK ½) | Cell Signaling | Western Blot | 1000 |
| S6 ribosomal protein | Cell Signaling | Western Blot | 1000 |
| p70 S6 kinase | Cell Signaling | Western Blot | 1000 |
| 4E-BP1 | Cell Signaling | Western Blot | 1000 |
| p21 cip1/waf1 | Cell Signaling | Western Blot | 1000 |
| Vimentin | Santa Cruz | IF | 100 |
| goat anti-rabbit | Pierce | 3000 | |
| goat anti-mouse | Pierce | 3000 | |
| mouse anti-goat | Pierce | 3000 | |
| Bax | Santa Cruz | IF | 100 |
IHC- Immunohistochemistry; IF- Immunofluorescence