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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: J Mol Biol. 2012 Oct 2;425(1):19–31. doi: 10.1016/j.jmb.2012.09.020

Figure 4.

Figure 4

The C-terminal 13 residues are necessary and sufficient for Pdr1 activation. Cells were transformed with vector containing DNA encoding TAP (dotted line) or TAP fused to fragments of the C-terminus of Zuo1, with the N-terminal residue of each fragment indicated. (a) Serial dilutions of wt cells harboring the indicated plasmids were spotted onto medium without (−) or with (+) cycloheximide. β-galactosidase activity was measured for cells containing an integrated PDR5-lacZ reporter and transformed with the indicated plasmids. The average activity of 3 transformants of each was quantified and reported in Miller units with error bars indicating standard error. (b) Serial dilutions of wt cells harboring the indicted plasmids were performed as described in a. (c) Cell extracts were prepared from cultures used for serial dilutions in (a) and (b) and subjected to immunoblot analysis using rabbit IgG to detect the TAP-tagged Zuo1 fusions.