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. Author manuscript; available in PMC: 2014 Jan 9.
Published in final edited form as: J Mol Biol. 2012 Oct 2;425(1):19–31. doi: 10.1016/j.jmb.2012.09.020

Figure 6.

Figure 6

Specificity of Zuo1’s hydrophobic C-terminus in Pdr1-dependent transcriptional activation. (left) Wt or Δpdr1 cells containing an integrated Gal1-HIS3 reporter were transformed with DNA encoding GBD (-) or GBD fused to one of three peptides: Zuo1421-433, P201, or P223. Cells were spotted in serial dilutions onto medium with (+) or without (−) histidine. (right) Wt or Δpdr1 cells were transformed with DNA encoding TAP (−) or TAP fused to the indicated peptides and spotted in serial dilutions onto medium without (−) or with (+) cycloheximide.