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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Free Radic Biol Med. 2012 Nov 1;54:1–16. doi: 10.1016/j.freeradbiomed.2012.10.553

Figure 5. IL-4-stimulated Stat6 activation is mediated by Jak1 whereas Tyk2 is required for Stat6 activation in IL-13-treated monocytes.

Figure 5

Monocytes (5×106/group) were pre-treated with scrambled/sense control ODNs or antisense ODNs to Jak1 (A, D) or Jak2 or Tyk2 (B, C) according to protocols described under “Materials and Methods” prior to the addition of IL-4 (A, B) or IL-13 (C, D) for 30 min. The cells were lysed and immunoblotted with anti-phospho-Stat6 antibody (upper panels of A–D). The same blots were stripped and reprobed with an antibody recognizing total Stat6 to confirm equal loading (lower panels of A–D). Data in panels A–D are from a representative of three repeat experiments showing similar results.