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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Res Microbiol. 2012 Oct 13;164(1):38–45. doi: 10.1016/j.resmic.2012.10.006

Fig. 2. Control of serralysin production by cAMP-CRP.

Fig. 2

A. Representative semi-quantitative RT-PCR of WT (CMS376) and crp (CMS1687) cultures including the 16S rDNA amplicon to indicate equal starting RNA concentrations. B. Immunoblot of normalized secreted fractions with anti-serralysin antibody. C. Quantification of anti-serralysin immunoblots with bands measured by densitometry. B–C. 1. WT with empty vector (pMQ131); 2. cyaA mutant with empty vector (pMQ131); 3. cyaA mutant with cyaA on a plasmid (pMQ157); 4. cyaA mutant grown with exogenous cAMP (5 mM); 5. crp mutant. Asterisks indicate a significant difference from WT with empty vector (pMQ131), ANOVA with Tukey’s post-test (p<0.05). The average and standard deviation of three independent experiments are shown.