Table 1. S. marcescens.
strains, plasmids and primers used in this study.
| Name | Description | Source |
|---|---|---|
| CMS376 | Wild-type strain PIC 361 (Presque Island Culture Collection) | (Kalivoda et al., 2008) |
| CMS524 | CMS376 with cyaA-2 mutation, transposon insertion | (Kalivoda et al., 2008) |
| CMS613 | CMS376 with crp-1 mutation, insertion mutation | (Kalivoda et al., 2008) |
| CMS786 | CMS376 with crp-23 mutation, transposon insertion | (Kalivoda et al., 2008) |
| CMS1687 | CMS376 with crp-34 mutation, deletion mutation | (Kalivoda et al., 2010) |
| JF1C6 | CMS613 with prtS::tn, transposon insertion | This study |
| JF1C6r | JF1C6 with crp gene restored to wild-type | This study |
| C23M13 | CMS with lipD::tn, transposon insertion | This study |
| JF2E10r | CMS376 with hyper-PrtS phenotype | This study |
| Plasmids | ||
| pBT20 | Mariner transposon-delivery vector | (Kulasekara et al., 2005) |
| pMQ131 | oripBBR1, ahpA-3, oriT, URA3, CEN6/ARSH4 | (Shanks et al., 2009) |
| pMQ157 | pMQ131 + cyaA | (Kalivoda et al., 2008) |
| pMQ166 | pMQ131 + crp | (Kalivoda et al., 2010) |
| Primer number | Sequence | Target |
| 2638 | AACTGGAGGAAGGTGGGGAT | 16S-rDNA |
| 2639 | AGGAGGTGATCCAACCGCA | 16S-rDNA |
| 2900 | TACAACGTTGCGCAGAACTC | chiA |
| 2901 | GTAGTCCACGATGCCGTTCT | chiA |
| 2903 | AAAACTTCCCGTACCCTGCT | chp21 |
| 2904 | GTTCGGCTTGGTGATGAAAT | chp21 |
| 2970 | CTCTCCGTAGAAGGCGTGAC | lipB |
| 2971 | GTTAGGGAAACGCAGGATCA | lipB |
| 2973 | TGTTCGCCTTTTTCGATACC | hasD |
| 2974 | TGTTCGCCTTTTTCGATACC | hasD |