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. Author manuscript; available in PMC: 2013 Aug 21.
Published in final edited form as: Circulation. 2012 Jul 11;126(8):963–974. doi: 10.1161/CIRCULATIONAHA.112.094722

Figure 5.

Figure 5

ET-1 stimulates PGC-1α-dependent association of SF with CYP11B2 to increase aldosterone levels. (a) The effect of ET-1 on PGC-1α expression was assessed by Western analysis (n=4). (b) Co-immunoprecipitation experiments demonstrated that incubation of HPAECs with ET-1 (10 nM) for 24 h induced the association of PGC-1α with steroidogenesis factor-1 (SF) (n=3). (c) Chromatin immunoprecipitation (n=3) of cell lysates using antibodies to PGC-1α, SF, and immunoglobulin-G (IgG) as a negative control was followed by PCR amplification of the proximal region of the CYP11B2 promoter region containing the gonadotrope-specific element. (d) The functional effect of PGC-1α stimulation on aldosterone production was assessed in cells treated with the selective PGC-1α agonist pioglitazone (50 μM) for 24 h (n=4), or with ET-1 (10 nM) or angiotensin II (ANG)(10 μM) for 24 h as positive controls. PGC-1α, PPAR-γ co-activator-1α; arb. units, arbitrary units; IP, immunoprecipitation, IB; immunoblot. Data are presented as mean ± S.E.M. Representative blots are shown.