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UV denaturation data of modified thrombin-binding aptamersa
| Oligonucleotide | Profile | Tm (°C) | ΔTm (°C) |
|---|---|---|---|
| TBA | I | 49.9 | -- |
| TBA-U7,U9 | I | 54.8 | +4.9 |
| TBA- FU7,FU9 | I | 51.9 | +2 |
| TBA-NT7,NT9 | II | 32.6 | −17.3 |
| TBA-U3,U4,U12,U13 | III | 32.2 | −17.7 |
| TBA-FU3,FU4,FU12,FU13 | III | 42.8 | −7.1 |
| TBA-U3,U4 | III | 41.0 | −8.9 |
| TBA-U4,U13 | II | 32.2 | −17.7 |
| TBA-NT4,NT13 | III | 31.0 | −18.9 |
Uncertainty ± 0.5° C, ODN concentration: 3 μM. Buffer. 10 mM sodium cacodylate 100 mM KCl pH 7
