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. 2012 Dec 13;10(1):68–72. doi: 10.7150/ijms.5256

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© Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited.

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Schematic mechanism of the WST-1 reduction. With intermediate electron acceptor (such as mPMS), the WST-1 reagent could be reduced to highly water soluble formazan by cellular dehydrogenases. Abbreviation: mPMS: 1-methoxy-5-methyl-phenazinium methyl sulfate; NADH: Nicotinamide adenine dinucleotide.