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. Author manuscript; available in PMC: 2013 Dec 28.
Published in final edited form as: Mol Cell. 2012 Nov 8;48(6):900–913. doi: 10.1016/j.molcel.2012.09.030

Figure 2. Changing cellular GCN5 levels alters Sirt6’s ability to change PGC-1α acetylation.

Figure 2

(A) Effects of GCN5 on Sirt6-mediated changes in PGC-1α acetylation. U-2 OS cultures were transfected with PGC-1α and increasing concentrations of Sirt6 with or without expression of WT GCN5. (B) Catalytically impaired GCN5 mutant, Y621A/F622A, fails to augment the Sirt6-mediated increase in PGC-1α acetylation (C) GCN5 knockdown diminishes the effect of Sirt6 on PGC-1α acetylation. (D) Co-IP of endogenous GCN5 with 3xFLAG Sirt6 from the nuclear fraction of U-2 OS cells. (E) Co-IP of endogenous Sirt6 with FLAG GCN5 from the nuclear fraction of U-2 OS cultures. (F) Co-IP of WT and HY Sirt6 with GCN5 from the nuclear fractions of U-2 OS cells.