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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Dev Biol. 2012 Oct 27;373(2):383–393. doi: 10.1016/j.ydbio.2012.10.023

Fig. 2. The Upd3 cytokine is required for stimulation of ISC division in response to challenge.

Fig. 2

(A) Structural organization of the upd3 locus in the parental strain (d00871) and in the upd3 deletion mutant (Δupd3#9). (B) pH3-positive nuclei were scored in wild-type (w1118), heterozygote (Δupd3#9/w1118), and upd3 mutant (Δupd3#9) animals infected for 18 hours with Erwinia carotovora caratovora 15 (Ecc15) (OD600=50), Pseudomonas entomophila (Pe), or Serratia marcescens (Sm). Averages of three representative experiments (n = 12 guts/experiment) with standard deviation are shown. P values were calculated by two-tailed, unpaired t-tests.

For Δupd3#9 vs. w1118: mock, P = 0.0437 (*); Ecc15 infection, P = 0.0015 (**); Pe infection, P = 0.0132 (*); Sm infection, P = 0.0010 (**).

For infection vs. mock, w1118: Ecc15, P =0.0044 (**); Pe, P = 0.0021 (**); Sm, P = 0.0066 (**).

For infection vs. mock, Δupd3#9: Ecc15, P =0.2698 (ns); Pe, P = 0.0111 (*); Sm, P = 0.7408 (ns).

(C) Genetic rescue of the upd3 mutant. pH3-positive nuclei were scored in wild-type (w1118), upd3 mutant (Δupd3#9) or rescued upd3 mutant animals (Δupd3#9;upd3>upd3: ectopic expression of UAS-Upd3 with upd3-GAL4, tub-Gal80ts). P values were calculated by two-tailed, unpaired t-tests. For mock infection, Δupd3#9 vs. w1118, P =0.2995 (ns) and wild-type vs. rescue, P<0.0001 (***). For Ecc15 infection, Δupd3#9 vs. w1118, P<0.0001 (***) and upd3>upd3 vs. w1118, P =0.38 (ns).