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. Author manuscript; available in PMC: 2014 Jan 15.
Published in final edited form as: Dev Biol. 2012 Oct 27;373(2):383–393. doi: 10.1016/j.ydbio.2012.10.023

Fig. 5. The Upd3 cytokine produced in EB locates to the extracellular matrix and activates JAK/STAT signaling in EB and in visceral muscles.

Fig. 5

(A) The Upd3-GFP fusion decorates muscles as visualized by F-actin staining.

(B) The Upd3-GFP fusion protein co-localizes with the extracellular matrix marker Viking.

(C) Magnification of the squared area shown in (B).

(D) The 10X-STAT-GFP reporter is expressed in EBs and display basal expression level in visceral muscles.

(E) Over-expression of the UAS-upd3 transgene using the Su(H)-Gal4ts driver leads to 10X-STAT-GFP expression in visceral muscles (asteriks) and differentiation of EBs (arrowhead pointing to the nucleus of a differentiating EBs).