Figure 3.
Bioprinted patch implementation. (a) Microscopic image of the mesenchymal stem cell (MSC) pattern directly after laser induced forward transfer (LIFT) printing. (b) fluorescence live/dead image of the printed cells after 6 days under chondrogenic culture conditions. (c) Image of MSCs, predifferentiated in osteogenic lineage for 7 days, printed in two chessboard patterns and cultivated for 17 days under osteogenic conditions. (d) Transmission image, (e) fluorescence images of nuclei stained with Hoechst 33342 (blue staining), and (f) membranes stained with DIL (red staining) of the chessboard section. Scale bar is 1 mm. (g) Arrangement of transferred cells by LIFT observed after 24 h: Human MSC were prestained with PKH26 and patches were stained with polyclonal goat anti-Pecam1 24 h after LIFT to separate grid patterned HUVEC. (h) Patch implantation in vivo: After LAD-ligation rats received the cardiac patch sutured onto the area of blanched myocardium. Reproduced with permission. Copyright Year, Publisher.