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. Author manuscript; available in PMC: 2014 Jan 20.
Published in final edited form as: Virology. 2012 Oct 12;435(2):415–424. doi: 10.1016/j.virol.2012.09.031

Fig. 5. rMP12-NSsR173A virus inhibits IFN-β mRNA synthesis.

Fig. 5

(A) MEF/wt cells or MEF/PKR0/0 cells were mock-infected or infected with MP-12, rMP12-C13type (C13type), rMP12-NSsR173A (R173A), or rMP12-mPKRN167 (N167), or (C) MRC-5 cells were mock-infected or infected with rMP12-rLuc, MP-12 or rMP12-NSsR173A virus at an moi of 3. Total RNA was harvested at 7 hpi. Northern blotting was performed with the strand-specific RNA probes to detect IFN-β or ISG56 mRNA, or RVFV anti-sense S-segment / N mRNA.