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. Author manuscript; available in PMC: 2014 Jan 20.
Published in final edited form as: Virology. 2012 Oct 12;435(2):415–424. doi: 10.1016/j.virol.2012.09.031

Fig. 7. R173A NSs-mediated eIF2α phosphorylation is decreased in the presence of dominant-negative PKR.

Fig. 7

(A) Human Hec1B cells were mock-infected or infected with rMP12-rLuc or rMP12-PKRΔE7 at an moi of 3. Then, cells were mock-transfected or immediately transfected with in vitro synthesized RNA encoding R173A NSs. Cells were collected at 16 hpi, and the abundance of PKR, phosphorylated eIF2α (Ser51), total eIF2α, R173A NSs, N and β-actin was analyzed by Western blot. (B) The relative abundance of phosphorylated eIF2α and NSs were analyzed. The density of phosphorylated eIF2α (normalized to total eIF2α) or NSs in mock-infected cells which are transfected with R173A NSs RNA is represented as 100%. The mean +/− standard deviation of three independent experiments is shown as a graph. Asterisk represents statistical significance (unpaired t-test, *p<0.05, **p<0.01).