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. Author manuscript; available in PMC: 2014 Jan 20.
Published in final edited form as: Virology. 2012 Oct 12;435(2):415–424. doi: 10.1016/j.virol.2012.09.031

Fig. 8. Cellular localization of MP-12 NSs and R173A NSs.

Fig. 8

MEF/PKR0/0 cells were mock-transfected or transfected with in vitro synthesized RNA encoding MP-12 NSs or R173A NSs. At 14 hours post transfection, cells were fixed with methanol, and indirect immunofluorescent assay using anti-RVFV antibody was performed. Mock-transfected cells (A) or cells transfected with MP-12 NSs RNA (B–D) or R173A NSs RNA (E and F) were incubated with anti-RVFV antibody (A and C–F) or normal mouse serum (B). Nuclei were visualized by DAPI. Scale (50 µm) is shown as while bars, and enlarged image is shown as an inset in E. White arrow(s) indicate the area unstained with DAPI with filamentous shadow (B), or cells corresponding to inset image (E).