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. Author manuscript; available in PMC: 2013 May 21.
Published in final edited form as: Cell. 2012 Nov 21;151(5):1042–1054. doi: 10.1016/j.cell.2012.10.044

Figure 4. Rqc1 levels are autoregulated by a conserved negative feedback loop.

Figure 4

(A) GFP levels in strains expressing a contranslationally degraded polybasic reporter subject to 10 hours cycloheximide treatment at the indicated concentration. (B) Ribosome footprint density at endogenous polybasic stretches (6 or greater K or R per 10 residues, N=103). (C) Conservation of Rqc1, with polybasic and TCF25 (Bateman et al., 2004) domains highlighted. (D) Assay showing the ability of Rqc1 alleles (WT, rqc1-FLAG, rqc1ala-FLAG, rqc1Δ) to act upon a model cotranslationally degraded substrate. (E) rqc1-FLAG and rqc1ala-FLAG protein levels in deletion strains. (F) Results of screen for regulators model polybasic substrate (full results in Table S1). tae2Δ and the four strongest hits labeled. (G) GFP and RFP levels of model polybasic substrate in selected hits from full-genome screen. Also see Table S4 and Figure S5.