| Component | Amount per reaction (μl) | Final |
|---|---|---|
| Ligation and primer | 12.0 | |
| First-strand buffer (5x) | 4.0 | 1x |
| dNTPs (10 mM) | 1.0 | 0.5 mM |
| DTT (0.1M) | 1.0 | 5 mM |
| SUPERase*In (20 U/μl) | 1.0 | 20 U |
| SuperScript III (200 U/μl) | 1.0 | 200 U |
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