| Component | Amount per reaction (μl) | Final |
|---|---|---|
| Phusion HF Buffer (5x) | 20 | 1x |
| dNTPs (10 mM) | 2.0 | 0.2 mM |
| Forward Library Primer (100 μM) | 0.5 | 0.5 μM |
| Reverse Indexed Primer (100 μM) | 0.5 | 0.5 μM |
| Circularized DNA template (from step 54) | 5.0 | |
| Nuclease-free water | 71.0 | |
| Phusion polymerase (2 U/μl) | 1.0 | 2 U |
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