Table 1. Primer and probe sequences for qMSP.
| Assay | Forward primer (5′–3′) | Reverse primer (5′–3′) | Probe | Refseq | Reference no. |
|---|---|---|---|---|---|
| ITGA4 | GCGGTTCGTATTCGGAGAAG | TCTACCGCCAACCGAAAACT | 6FAM-AGCGCGAGTATTC-MGB | NM_000885 | 19 |
| NTRK2 | CGTTGGTTGCGTATTTGGC | GCAATACCTAACACTTCCGAAAACTC | 6FAM-TGTTCGTCGCGATTC-MGB | NM_006180 | 20 |
| OSMR | CGTCGGTTGGTTCGTGC | CCGAACTTTACGAACGAACGA | 6FAM-CGGTCGTCGAGTTTT-MGB | NM_03999 | 20 |
| TUBG2 | TAACGAGTTTTATTGCGTAGGCG | CAACTAAAATTCCGCACAAACG | 6FAM-GGAGCGGGCGTGGT-MGB | NM_016437 | 20 |
| SEPT9 | CGCGCGATTCGTTGTTTATTA | CCAACCCAACACCCACCTT | 6FAM-GGATTTCGCGGTTAAC-MGB | NM_001113493 | 6 |
| VIM | GGTCGAGTTTTAGTCGGAGTTACGT | CCCGAAAACGAAACGTAAAAACTA | 6FAM-CGTATTTATAGTTTGGGTAGCGC-MGB | NM_003380 | 18 |
MGB, minor groove binder; qMSP, quantitative methylation-specific PCR.