Table II.
Immunoinhibition of P450-Mediated AA Epoxygenation and Hydroxylation in Rat Heart, Lung, Kidney, and Liver Microsomes
| Antibody | % inhibition | |||||||
|---|---|---|---|---|---|---|---|---|
| Heart | Lung | Kidney | Liver | |||||
| Epoxygenation | Hydroxylation | Epoxygenation | Hydroxylation | Epoxygenation | Hydroxylation | Epoxygenation | Hydroxylation | |
| Anti-CYP2B1/2 | 4.48 ± 3.63 | 8.20 ± 4.17 | 46 ± 14 | 20.5 ± 3.9 | 11.2 ± 5.5 | 21.5 ± 11.6 | 14.5 ± 9.8 | 8.00 ± 9.43 |
| Anti-CYP2C11 | 89 ± 25 | 11 ± 5 | 17.5 ± 6.6 | 15.2 ± 6.8 | 58.1 ± 22.8 | 25.4 ± 21.9 | 88.5 ± 26.5 | 30.2 ± 7.1 |
| Anti-CYP2C23 | 5.02 ± 3.66 | 8.87 ± 4.18 | 9.51 ± 6.02 | 3.02 ± 2.38 | 72.5 ± 16.9 | 4.73 ± 6.93 | 62.6 ± 28 | 27.3 ± 8.4 |
| Anti-CYP4A1/2/3 | 17.5 ± 4.2 | 76 ± 28 | 23 ± 10 | 23.9 ± 15.8 | 27.7 ± 23.7 | 71.5 ± 27.3 | 21.4 ± 6.1 | 35.1 ± 10.1 |
Data are the mean of at least three determinations and SEM